The seminar will be focused on application new, plasma technique for deposition of enzyme bio-recognition layers and in consequence biosensor construction. This technique called Soft Plasma Polymerization (SPP) is based on low energy density Corona Discharge of plasma generated between two spark electrodes in presence of Helium carrier gas. Plasma deposition process of enzyme bio-recognition layer is consisted of two steps: (I) polymerization/cross-linking biological precursor inside plasma zone and (II) binding of polymerized/cross-linked biologically active molecules. So far I carried out optimization of plasma parameters (voltage, helium carrier gas flow rate) and process parameters (deposition time, biological precursor flow rate) optimization for 3 solid supports i.e. glass slides, graphene oxide and multi-walled carbon nanotubes. Then, I applied SPP technique in construction of laccase-based electrochemical biosensors. Constructed biosensors were applied in determination pharmaceutics i.e. rutin and dopamine and environmental pollutions i.e. catechol, hydroquinone and resorcinol. The electrochemical measurement showed that application of SPP technique allows on significant reduction time needed to construction without losing analytical parameters of devices. Therefore, the SPP technique has high commercial value because can trade up to currently ways of biosensors construction called “wet” methods. As literature indicates “wet” methods are complex, time-consuming and in many cases needs long-hour conditioning of transducer element in enzyme solution in order to deposit of bio-recognition layer.